OAR@UM Community:

MRI perfusion of solitary parotid lesions : a prospective study

2026-05-11T12:21:36Z

Title: MRI perfusion of solitary parotid lesions : a prospective study Abstract: Background: Parotid tumours (PTs) have a variety of pathological subtypes, each requiring distinct surgical approaches. Nevertheless, the current preoperative diagnostic methods often fall short in providing accurate PT diagnoses. Aim: Can DCE-MRI help predict histology in patients with focal parotid lesions? Design: A prospective study recruiting patients with known focal lesions of the parotid gland who are being worked up for surgery. A dynamic contrast-enhanced MRI (DCE-MRI) scan is performed, after which post-processing of data is used to obtain MR perfusion quantitative parameters (Ktrans, Kep and Ve) obtained by drawing a region of interest (ROI) over the PT. In this study 2 individual sets ROIs were drawn by two authors. The first set of ROIs vary in size, whereas the second are a fixed range ROI size limited to 10-20mm². The information obtained from DCE-MRI is compared to tissue histology and cytology, to assess the diagnostic accuracy of the DCE-MRI. The intra and inter-observer correlation of the different ROI methods is also assessed. Ethical standards were adhered to, with informed consent obtained from all participants. The data collection and analysis process spanned approximately 10 months. Methods: In this prospective study, 15 patients with histologically or cytologically proven PTs who underwent DCE-MRI were enrolled including 7 pleomorphic adenomas (PAs) and 8 Warthin’s tumours (WTs). Quantitative parameters of DCE-MRI (Ktrans, Kep, and Ve) of lesions were calculated and analysed. A second set of analysis was done to assess the intra and inter-observer correlation when using different ROI techniques. Results: Statistically significant (p < 0.05) inter-group differences were found between PA and WT for Kep and Ve. Correlation with histological results using the Mann-Whitney U test, where Ktrans, Kep and Ve were found to have p-values of 0.132, 0.008 and 0.003 respectively. Inter-observer correlation for the first set of variable size ROIs for the different MRI parameters were: Ktrans 0.590, Kep 0.622 and Ve 0.848, and for the second fixed range set Ktrans 0.945, Kep 0.901 and Ve 0.964. The intra-observer results for author 1 for the different MRI parameters were: Ktrans 0.358, Kep 0.718 and Ve 0.436. The intra-observer results for author 2: Ktrans 0.950, Kep 0.581 and Ve 0.762. Conclusions: In summary, DCE-MRI perfusion parameters aid in the differentiation of PA and WT. This technique demonstrates reliability and reproducibility. Standardised ROI sizes with fixed ranges minimise the impact of radiologist experience on perfusion parameter calculations, resulting in better interobserver agreement. However, when evaluating the reproducibility of perfusion parameters obtained by the same reader using different ROI techniques, radiologist experience significantly affects parameter calculations. Description: M.Sc.(Melit.)

The use of shear-wave elastography in differentiating renal cell carcinoma and renal oncocytoma

2026-05-11T12:18:11Z

Title: The use of shear-wave elastography in differentiating renal cell carcinoma and renal oncocytoma Abstract: Background: Renal tumours pose a significant diagnostic challenge, particularly in distinguishing malignant renal cell carcinomas (RCCs) from benign renal oncocytomas (ROs). Aim: This study aimed to evaluate the efficacy of shear wave elastography (SWE) in characterising RCCs and ROs based on tissue stiffness. Methods: Conducted at Mater Dei Hospital, Malta, from September to November 2024, this prospective study included 33 participants presenting 36 renal lesions. SWE was performed using a Canon Aplio a550 ultrasound system, and quantitative elasticity metrics were analysed to differentiate between RCCs and ROs. Results: Mann-Whitney U tests proved no statistical significant difference between RCCs and ROs. Mean speed measured for ROs was 2.43m/s ± 0.72m/s and 2.87m/s ± 0.83m/s for RCCs. Area under the curve (AUC) in receiver operator characteristic (ROC) curve analysis mean speed m/s was 0.660. SWE demonstrated no reliability in differentiating between RCCs and ROs. Conclusion: The results highlight the need for research into this field and emphasise the need for standardisation of methodology and development and further study of SWE in differentiating renal tumours with greater precision and confidence. Description: M.Sc.(Melit.)

Diagnostic accuracy of proton magnetic resonance spectroscopy for non-invasive detection of 2-hydroxyglutarate and IDH mutation status in gliomas : a meta-analysis

2026-05-11T12:09:09Z

Title: Diagnostic accuracy of proton magnetic resonance spectroscopy for non-invasive detection of 2-hydroxyglutarate and IDH mutation status in gliomas : a meta-analysis Abstract: Isocitrate dehydrogenase (IDH) mutation status is a central determinant of modern glioma classification, prognosis, and therapeutic decision-making. Mutant IDH enzymes acquire a neomorphic catalytic function that leads to the pathological accumulation of the oncometabolite D-2-hydroxyglutarate (2-HG), a metabolite that is largely absent in IDHwildtype gliomas and normal brain tissue. This mutation-specific metabolic alteration provides a unique opportunity for non-invasive molecular characterisation, as intracellular 2- HG accumulates to concentrations detectable in vivo using proton magnetic resonance spectroscopy (MRS). The recent approval of IDH-targeted therapy following the phase III INDIGO trial has further increased the clinical importance of early and accurate identification of IDH mutation status. This dissertation aimed to evaluate the diagnostic accuracy of in vivo proton MRS for 2-HG detection as a non-invasive biomarker of IDH1/IDH2 mutation in gliomas, and to assess whether diagnostic performance has changed over time in parallel with methodological advances in MRS acquisition and analysis. A systematic review and meta-analysis were conducted in accordance with PRISMADTA guidelines. PubMed, MEDLINE, and Scopus were searched for studies published between 2012 and 2023 that evaluated in vivo proton MRS for 2-HG detection in human glioma cohorts with histopathologically confirmed IDH status. Studies providing sufficient data to construct 2×2 contingency tables were included. Methodological quality was assessed using the QUADAS-2 tool. Pooled sensitivity and specificity were estimated using a bivariate random-effects model, and overall diagnostic performance was summarised using a summary receiver operating characteristic curve. A predefined temporal subgroup analysis compared studies published up to and including 2018 with those published after 2018. Twenty-one studies met the inclusion criteria. Proton MRS demonstrated high diagnostic accuracy for identifying IDH mutation status, with pooled sensitivity of 86.8% and pooled specificity of 84.3%, and an area under the summary receiver operating characteristic curve of 0.91. Diagnostic performance remained stable over time, with a trend toward improved specificity in more recent studies, consistent with increasing methodological standardisation and clinical translation. Risk of bias was low to moderate across studies, predominantly related to patient selection and diagnostic thresholds. In conclusion, proton MRS enables reliable, non-invasive detection of 2- hydroxyglutarate and accurate prediction of IDH mutation status in gliomas. In the context of newly approved IDH-targeted therapies, these findings support the role of 2-HG MRS as a clinically meaningful adjunct to tissue-based molecular diagnostics, particularly in patients for whom surgical sampling is limited or high-risk. Description: M.Sc.(Melit.)

Malignant transformation of hypointense nodules seen only on hepatobiliary phase gadoxetic acid-enhanced MR imaging : a systematic review and meta-analysis

2026-05-11T12:07:19Z

Title: Malignant transformation of hypointense nodules seen only on hepatobiliary phase gadoxetic acid-enhanced MR imaging : a systematic review and meta-analysis Abstract: Background: Hypointense nodules detected on the hepatobiliary phase (HBP) of gadoxetic acid–enhanced magnetic resonance imaging (MRI) are frequently seen in patients with chronic liver disease or cirrhosis. Such nodules may represent a spectrum from dysplasia to early hepatocellular carcinoma (HCC), and the development of arterialphase hyperenhancement and subsequent hypervascular transformation of these lesions is indicative of an increased malignant potential. Aim: Through a comprehensive systematic review and meta-analysis, this study seeks to evaluate the outcomes of hypointense nodules observed in the hepatobiliary phase on gadoxetic acid-enhanced MRI and to identify the risk factors associated with their hypervascular transformation. Methods: A computerised search of the Pubmed, Web of Science and Scopus databases was carried out to identify relevant literature reports on hypovascular hypointense nodules in the hepatobiliary phase of gadoxetic acid–enhanced MRI that will eventually undergo hypervascular transformation. Results: 18 studies were included in this meta-analysis, with 1563 patients and a total of 2136 hypointense hypovascular hepatic nodules. The pooled overall rate of hypervascular transformation was 34.4%, (95% CI: [28.7%, 40.2%]) and I2 was 87.4%. The pooled 1-, 2- and 3-year cumulative incidence rates were 19.7% (95% CI: [9.1%, 30.4%]), 27.9%, (95% CI: [16.3%, 39.5%]); and 27.2% (95% CI: [17.7%, 36.7%], respectively. A metaregression analysis revealed that the heterogeneity of malignant transformation was significantly affected by the initial mean nodule size, with a cutoff value of 9 mm. Conclusion: Hypovascular hypointense nodules observed during the hepatobiliary phase of Gadoxetic acid-enhanced MRI present a considerable risk of progressing to hypervascular hepatocellular carcinomas (HCCs). Initial nodule size is an important predictor of hypervascular transformation, with larger nodules being more likely to undergo this process. This highlights the importance of close monitoring, to enable timely intervention to improve patient outcomes. Description: M.Sc.(Melit.)