/library/oar/handle/123456789/691 2025-11-10T18:31:58Z 2025-11-10T18:31:58Z Vella, Nathan Fenech, Anthony G. Petroni Magri, Vanessa /library/oar/handle/123456789/138976 2025-09-12T09:18:47Z 2024-01-01T00:00:00Z

Title: 3D cell culture models in research : applications to lung cancer pharmacology Authors: Vella, Nathan; Fenech, Anthony G.; Petroni Magri, Vanessa Abstract: Lung cancer remains one of the leading causes of cancer-related mortality worldwide, necessitating innovative research methodologies to improve treatment outcomes and develop novel strategies. The advent of three-dimensional (3D) cell cultures has marked a significant advancement in lung cancer research, offering a more physiologically relevant model compared to traditional two-dimensional (2D) cultures. This review elucidates the various types of 3D cell culture models currently used in lung cancer pharmacology, including spheroids, organoids and engineered tissue models, having pivotal roles in enhancing our understanding of lung cancer biology, facilitating drug development, and advancing precision medicine. 3D cell culture systems mimic the complex spatial architecture and microenvironment of lung tumours, providing critical insights into the cellular and molecular mechanisms of tumour progression, metastasis and drug responses. Spheroids, derived from commercialized cell lines, effectively model the tumour microenvironment (TME), including the formation of hypoxic and nutrient gradients, crucial for evaluating the penetration and efficacy of anti-cancer therapeutics. Organoids and tumouroids, derived from primary tissues, recapitulate the heterogeneity of lung cancers and are instrumental in personalized medicine approaches, supporting the simulation of in vivo pharmacological responses in a patient-specific context. Moreover, these models have been co-cultured with various cell types and biomimicry extracellular matrix (ECM) components to further recapitulate the heterotypic cell-cell and cell-ECM interactions present within the lung TME. 3D cultures have been significantly contributing to the identification of novel therapeutic targets and the understanding of resistance mechanisms against conventional therapies. Therefore, this review summarizes the latest findings in drug research involving lung cancer 3D models, together with the common laboratory-based assays used to study drug effects. Additionally, the integration of 3D cell cultures into lung cancer drug development workflows and precision medicine is discussed. This integration is pivotal in accelerating the translation of laboratory findings into clinical applications, thereby advancing the landscape of lung cancer treatment. By closely mirroring human lung tumours, these models not only enhance our understanding of the disease but also pave the way for the development of more effective and personalized therapeutic strategies.

2024-01-01T00:00:00Z Zancolli, Giulia von Reumont, Björn Marcus Anderluh, Gregor Caliskan, Figen Chiusano, Maria Luisa Fröhlich, Jacob Hapeshi, Evroula Hempel, Benjamin-Florian Ikonomopoulou, Maria P. Jungo, Florence Marchot, Pascale Mendes de Farias, Tarcisio Modica, Maria Vittoria Moran, Yehu Nalbantsoy, Ayse Procházka, Jan Tarallo, Andrea Tonello, Fiorella Vitorino, Rui Zammit, Mark Lawrence Antunes, Agostinho /library/oar/handle/123456789/138969 2025-09-12T08:51:33Z 2024-01-01T00:00:00Z

Title: Web of venom : exploration of big data resources in animal toxin research Authors: Zancolli, Giulia; von Reumont, Björn Marcus; Anderluh, Gregor; Caliskan, Figen; Chiusano, Maria Luisa; Fröhlich, Jacob; Hapeshi, Evroula; Hempel, Benjamin-Florian; Ikonomopoulou, Maria P.; Jungo, Florence; Marchot, Pascale; Mendes de Farias, Tarcisio; Modica, Maria Vittoria; Moran, Yehu; Nalbantsoy, Ayse; Procházka, Jan; Tarallo, Andrea; Tonello, Fiorella; Vitorino, Rui; Zammit, Mark Lawrence; Antunes, Agostinho Abstract: Research on animal venoms and their components spans multiple disciplines, including biology, biochemistry, bioinformatics, pharmacology, medicine, and more. Manipulating and analyzing the diverse array of data required for venom research can be challenging, and relevant tools and resources are often dispersed across different online platforms, making them less accessible to nonexperts. In this article, we address the multifaceted needs of the scientific community involved in venom and toxin-related research by identifying and discussing web resources, databases, and tools commonly used in this field. We have compiled these resources into a comprehensive table available on the VenomZone website (https://venomzone.expasy.org/10897). Furthermore, we highlight the challenges currently faced by researchers in accessing and using these resources and emphasize the importance of community-driven interdisciplinary approaches. We conclude by underscoring the significance of enhancing standards, promoting interoperability, and encouraging data and method sharing within the venom research community.

2024-01-01T00:00:00Z Da Costa, Filipa Alves Fernández-Llimós, Fernando Desselle, Shane Arnet, Isabelle Babar, Zaheer Bond, Christine Cordina, Maria Cardenas, Victoria Garcia El Hajj, Maguy S. Jacobsen, Ramune Law, Anandi V. Nørgaard, Lotte S. Polidori, Carlo Shcherbakova, Natalia Stewart, Derek Tonin, Fernanda S. Weidmann, Anita E. /library/oar/handle/123456789/138141 2025-08-18T09:12:19Z 2025-01-01T00:00:00Z

Title: La International collaboration of pharmacy journal editors (ICPJE) se constituye formalmente para fomentar la calidad en torno a las publicaciones de investigación sobre farmacia práctica, clínica y social Authors: Da Costa, Filipa Alves; Fernández-Llimós, Fernando; Desselle, Shane; Arnet, Isabelle; Babar, Zaheer; Bond, Christine; Cordina, Maria; Cardenas, Victoria Garcia; El Hajj, Maguy S.; Jacobsen, Ramune; Law, Anandi V.; Nørgaard, Lotte S.; Polidori, Carlo; Shcherbakova, Natalia; Stewart, Derek; Tonin, Fernanda S.; Weidmann, Anita E. Abstract: The Granada statements were a result of the need to strengthen clinical, social and administrative pharmacy practice as an area of knowledge that translates into practice, research and policy. As a response, a group of clinical and social pharmacy practice journal editors launched an initiative in Granada in 2022 to discuss ways to improve the quality of publications in this area, which culminated in the Granada statements. Eighteen statements were developed, clustered into six main domains:1) the appropriate use of terminology; 2) developing impactful abstracts; 3) having the required peer reviews; 4) preventing journal scattering; 5) more effective and wiser use of journal and article performance metrics; and 6) authors’ selection of the most appropriate pharmacy practice journal to submit their work. The full Granada statements have been published in 14 journals. These pioneering statements are rooted in similar endeavors undertaken by scholars in other health professions groups, fostering the concept of interdisciplinary consensus and advancing scientific paradigm.

2025-01-01T00:00:00Z Babey, Anna-Marie Koenig, Jennifer Cunningham, Margaret Shield, Alison Restini, Carolina Djouma, Elvan Mraiche, Fatima Mifsud, Janet Kelly, John P. Nicolazzo, Joseph Karpa, Kelly J. Volbrecht, Kieran Santiago, Marina Junqueira Hawes, Martin Aljofan, Mohamad Kelly-Laubscher, Roisin Karunaratne, Nilushi Tucker, Steven J. Hinton, Tina Liang, Willmann Guilding, Clare White, Paul J. /library/oar/handle/123456789/134706 2025-04-28T08:21:47Z 2025-01-01T00:00:00Z

Title: Evaluating student understanding of core pharmacokinetic concepts Authors: Babey, Anna-Marie; Koenig, Jennifer; Cunningham, Margaret; Shield, Alison; Restini, Carolina; Djouma, Elvan; Mraiche, Fatima; Mifsud, Janet; Kelly, John P.; Nicolazzo, Joseph; Karpa, Kelly J.; Volbrecht, Kieran; Santiago, Marina Junqueira; Hawes, Martin; Aljofan, Mohamad; Kelly-Laubscher, Roisin; Karunaratne, Nilushi; Tucker, Steven J.; Hinton, Tina; Liang, Willmann; Guilding, Clare; White, Paul J. Abstract: Both educators and graduates have expressed concern about a perceived pharmacology knowledge gap that includes difficulty applying fundamental principles to clinical and research problems. Consequently, we sought to determine the extent to which current students can explain the meaning of, and appropriately apply, a subset of core concepts, and to identify any misconceptions arising from the responses. Of the twenty-four pharmacology core concepts arising from the recent international collaboration, four pharmacokinetic concepts were chosen, namely drug bioavailability, drug clearance, volume of distribution, and steady-state concentration. A total of 318 students from 11 universities across seven countries chose to participate in this study. Expert analysts identified the essential elements for each concept, then independently assessed each student's response. Teams of two experts compared their evaluations to reach a consensus and grouped misconceptions thematically. For each core concept, less than 30% of students provided responses that encompassed all essential elements. Participants found drug clearance most challenging, generally conflating it with the rate of elimination, whereas they demonstrated a better understanding of drug bioavailability. There were 34 misconception themes coded in a total of 813 statements, with volume of distribution and drug clearance producing the highest numbers (13 and 12, respectively). Overall, results suggest that students found it easier to apply the concept than to explain its meaning, which might reflect the shift from didactic to active learning approaches. These findings may be useful for educators who are developing introductory pharmacokinetic courses by providing conceptual focus and revealing common misconceptions to explicitly address.

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