OAR@UM Collection: /library/oar/handle/123456789/77818 2025-12-26T11:09:20Z 2025-12-26T11:09:20Z Cloning of Escherichia Coli iron superoxide dismutase in yeast cells deficient in copper, zinc superoxide dismutase /library/oar/handle/123456789/77757 2024-02-09T11:09:11Z 1996-01-01T00:00:00Z Title: Cloning of Escherichia Coli iron superoxide dismutase in yeast cells deficient in copper, zinc superoxide dismutase Abstract: An episomal and a centromeric plasmid were used in the cloning of ‘Esherichia coli’ iron superoxide dismutase (FeSOD) in ‘Saccharomyces cervisiae’ cells deficient in copper, zinc superoxide dismutase (Cu,ZnSOD). In both cases the ‘E.coli’ FeSOD gene was unde the transcriptional control of the yeast PGK promoter. The prokaryotic FeSOD was shown to be functionally expressed at different levels within the two recombinant strains. Moreover, the FeSOD activity was found to decrease as the cells approached stationary phase in both strains. This decrease is attributed to the decrease in glucose concentration within the fixed volume medium. Description: M.PHIL. 1996-01-01T00:00:00Z